Day 1 :
Baylor College of Medicine, USA
Time : 09:45-10:25
Nathan S Bryan is an international expert on nitrate, nitrite and nitric oxide. He has made many seminal discoveries in the field of nitric oxide. These discoveries and findings have unveiled many beneficial effects of nitrite in the treatment and prevention of human disease and may provide the basis for new preventive or therapeutic strategies in diseases associated with NO insufficiency and new guidelines for optimal health. He has published a number of highly cited papers and authored or edited 5 books. He is also an inventor on multiple issued patents.
There are now indisputable health benefits of nitrite when administered in a clinical setting for specific diseases. Most of the published reports identify the production of nitric oxide (NO) as the mechanism of action for nitrite. Basic science as well as clinical studies demonstrates nitrite and/or nitrate can restore NO homeostasis as an endothelium independent source of NO that may be a redundant system for endogenous NO production. Nitrate must first be reduced to nitrite by oral commensal bacteria and then nitrite further reduced to NO along the physiological oxygen gradient. Despite decades of rigorous research on its safety and efficacy as a curing agent, sodium nitrite and nitrate are still regarded by many as a toxic undesirable food additive. However, research within the biomedical science community has revealed enormous therapeutic benefits of nitrite and nitrate that are currently being developed as novel therapies for conditions associated with nitric oxide insufficiency. This presentation will highlight the fundamental biochemistry of nitrite and nitrate in human physiology and provide evidence that nitrite and nitrate be considered essential nutrients. Foods or diets enriched with nitrite can have profound positive health benefits.
La Trobe University, Australia
Keynote: Inclusion of molasses in a high fat, high sugar diet prevents the development of obesity in C57BL6/J mice
Time : 10:45-11:25
Markandeya Jois has expertise in the prevention and treatment of obesity and metabolic syndrome. His research in this area employs animal models as well as clinical trials with particular emphasis on the role of food plants in disease prevention
Statement of the problem: One of the contributing factors for the current epidemic of obesity is the increased consumption of added sugar in the diet. Sugarcane accounts for 80% of the sugar production. Molasses, a byproduct of sugar production from sugarcane, is known rich in many bioactive compounds including flavonoids and phenylpropanoids. Here we show that inclusion of molasses in a high fat, high sugar diet protects mice from developing obesity. Methods: Thirty-six 6-wk old male mice were assigned to one of 3 groups. Each group was fed either a high fat, high sugar diet (HFC), a HFC diet containing 10% molasses replacing 5% sugar and 5% cellulose (Mol-Sugar) or a HFC diet containing 10% molasses replacing 5% starch and 5% cellulose (Mol-Starch). Mice were maintained on these diets for 14 weeks and food intake and bodyweights were monitored weekly. At weeks 7 and 14, randomly selected mice were culled and body composition, plasma metabolites and tissue gene expression were measured Findings: Molasses groups gained significantly less bodyweight and had lower body fat. The effects of molasses on body fat content was greater in the Mol-Sugar group. Reduced body fat in the molasses groups were associated with an up-regulation of SIK2 gene expression and a down-regulation of ATF3 gene expression in adipose tissue. Circulating adiponectin levels were also elevated in the molasses groups. Conclusion & Significance: Sugarcane molasses contain anti-obesity compounds and addition of these compounds to sugar containing food products may help prevent the development of obesity.
Saveetha Medical College, India
Time : 11:25-12:05
Krishnan Vengadaragava Chary did his post graduation from prestigious Stanley Medical College. He is an eminent pharmacologist , well known for his teaching skills in college and PG entrance coaching. He has won many prizes including a best poster in IPS conference at Bangalore. He is research coordinator of Saveetha Medical College and consultant for few ethics committee and contract research organization at Chennai. He has five SCOPUS , PUBMED indexed publications. He is reviewer of Science and Engineering Research Board ( SERB), Govt of India and in few biomedical journals. He has authored a chapter of Pharmacology in book entitled TARGET JIPMER , 1st ed, 2013 published by Wolters Kluwer
- Special Session
Location: Las Vegas
- Oral Session
Location: Las Vegas
La Trobe University, Australia
St. Philip College, USA
U.S. Food and Drug Administration, USA
Title: Bio-Plex suspension array immuno-detection of Listeria monocytogenes from lettuce and spinach using virulence protein inducing charcoal-activated enrichment media
Time : 12:05-12:25
James B Day is a research microbiologist at the U. S Food and Drug Administration in College Park, Maryland where he is involved in developing detection methodologies for bacterial pathogens in contaminated foods. He has developed techniques for rapid identification of Francisella tularensis, Salmonella enterica and Listeria monocytogenes in various food matrices and recently established a novel macrophage-based assay for enrichment of intracellular bacterial pathogens for enhanced identification. He earned his Ph. D from the University of Miami School of Medicine (UM) where he worked on bacterial pathogenesis of Yersinia pestis. At UM, he developed a widely used system to measure virulence protein secretion and host cell translocation. He went on to complete his postdoctoral studies at Harvard Medical School where he worked on type III secretion mechanisms of Salmonella enterica as well as regulatory factors that control virulence protein induction.
Statement of the problem: Listeria monocytogenes, the causative agent of Listeriosis in humans, is a Gram-positive bacterium that is contracted via the consumption of contaminated foods. Various leafy green vegetables, including lettuce and spinach, have been implicated in human Listeriosis cases. Molecular methods and immuno-based techniques for detection of L. monocytogenes in these food matrices can be difficult due to the presence of assay inhibiting elements. Methodology: In this study, we utilize a novel enrichment media containing activated charcoal as the key ingredient that induces overexpression and secretion of L. monocytogenes virulence proteins. The Bio-Plex suspension array system, based on Luminex xMAP technology, can then be utilized to specifically detect accumulated L. monocytogenes virulence proteins via a magnetic bead-antibody complex. Iceburg lettuce and packaged ready-to-eat spinach were treated with L. monocytogenes and incubated in preenrichment broth (Buffered Listeria Enrichment Broth) followed by incubation in charcoal activated media. The supernatant fraction was TCA precipitated and L. monocytogenes lysteriolysin O (LLO) was collected using magnetic microspheres conjugated to LLO specific antibody. A newly develop antibody that exclusively recognize L. monocytogenes LLO was used as the biotin conjugated secondary antibody and analysis was conducted using the Bio-Plex 200 system. Findings: As few as 1 CFU/ g of L. monocytogenes was detected in both foods tested. Whole cell fractions from 14h activated charcoal enrichments were also analyzed using antibody that recognize both pathogenic and non-pathogenic Listeria species which also resulted in a detection limit of 1 CFU/ g. Internal control beads were also utilized to ensure proper instrumentation function, integrity of assay reagents and to eliminate the possibility of non-specific interactions. Conclusions and significance: This method is the first to specifically recognize and differentiate L. monocytogenes among other nonpathogenic Listeria species in various leafy greens using immune-detection.The total presumptive detection time can be achieved in less than 24h.
University of the Punjab, Pakistan
Time : 12:25-12:45
Nayab Batool Rizvi has her expertise in Clinical Biochemistry. Her main area of interest is Biochemistry and Food and Nutrition. She has done a lot of work on antioxidants and currently working as an Assistant Professor in the Institute of Chemistry (Biochemistry Section) University of the Punjab, Lahore, Pakistan. She has supervised more than 10 graduate and post-graduate students.
Edible oils are widely used throughout the world. Deep-fat frying is the most oldest and popular food preparation method around the globe. The study is undertaken to observe deep-fat frying phenomenon performed at high temperatures under atmospheric pressure. Deep frying results in deterioration of physical, chemical, nutritional and sensory properties of oil, which ultimately affects our health. It also results in the production of volatile products such as aldehydes and non-volatile fraction which remains in the frying medium. Some of these remaining products have been implicated in producing adverse health effects. Highly oxidized oils may also produce poly aromatic hydrocarbons, which have carcinogenic effect. This work was intended to evaluate the effect of frying on chemical properties of edible vegetable oils. Sunflower oil, olive oil and canola oil were used to fry French fries. Acid value, iodine value, peroxide value, saponification value and total polar compounds were measured by the standard ISO methods and traditional methods of determining these parameters. Acid values of all oils used were determined, that ranged between 0.3 and 28 (mg of KOH/g) but least value was of canola oil i.e., 0.561. Similarly, least iodine value is of fresh olive oil i.e., 75.94 and the highest value was obtained in multiple times used sunflower oil. Peroxide value of canola oil was least i.e., 4 (meqO2/kg) and the highest value was multiple times used canola oil. The results showed that all these parameters in all the oil types increased linearly with frying time. The influence of oil type on the content of total polar compounds, peroxide and acid value in used oil was significant, but the effect of food type on these parameters was not observed.
Tamil Nadu Agricultural University, India
Time : 12:45-13:05
Varadharaju N, PhD, is having expertise in reducing the post-harvest losses in perishables, for which he has contributed and established a Food Processing Business Incubator at the Post Harvest Technology Centre, TNAU, Coimbatore. His contributions in the development of processing machinery are noteworthy to mention. He has operated three international and four national research projects. He was instrumental in design and development of double chamber centrifugal de-huller for millets, for which he was conferred with a national award. He has got three decades of teaching and research experience in the field of Food and Agricultural Process Engineering. He has published 25 international and 40 national research papers in reputed journals.
Statement of the Problem: Investigating the effect of de-hulling process on milling and nutritional quality of millets. Millet grain is highly nutritious with good quality protein, rich in minerals, dietary fiber, phyto-chemicals and vitamins. The milling characteristics and retention of nutrients in the de-hulled millets depends on the process of de-hulling and the type of machinery employed.
Methodology & Theoretical Orientation: De-hulled millets obtained from the abrasive type mill and centrifugal de-huller was analyzed for recovery, broken, nutritional quality and shelf life under ambient conditions.
Findings: The study reveals that the recovery of de-hulled millet was around 10% more in centrifugal type (where the bran is retained) with 95% de-hulling efficiency compared to abrasive mill. The breakage was 4-5% in centrifugal type and there was only 1-2% in abrasive type. The nutritional content (carbohydrate, protein, fat, ash, calcium, phosphorus, iron and fiber) of the all five de-hulled millets (little millet, proso millet, foxtail millet, barnyard millet and Kodo millet) tested were resulting in superior quality in centrifugal de-huller.
Conclusion & Significance: The shelf life of de-hulled millets obtained from the abrasive mill was four times more than that of centrifugal type due to lesser amount of fat presence.
Saveetha Medical College, India
Time : 14:05-14:25
Krishnan Vengadaragava Chary Did his post graduation from prestigious Stanley Medical College . He is an eminent pharmacologist , well known for his teaching skills in college and PG entrance coaching. He has won many prizes including a best poster in IPS conference at Bangalore. He is research coordinator of Saveetha Medical College and consultant for few ethics committee and contract research organization at Chennai. He has five SCOPUS , PUBMED indexed publications. He is reviewer of Science and Engineering Research Board ( SERB), Govt of India and in few biomedical journals. He has authored a chapter of Pharmacology in book entitled TARGET JIPMER , 1st ed, 2013 published by Wolters Kluwer
The significance is that these irrational combinations of Vitamins have their own complex effects in the body and many drug-kinetics interactions. These FDCs are freely available and widely consumed by consumers under over the counter in India. The objective of our study to analyze the rationality of Vitamins and minerals combinations available in India.; to provide comprehensive data of irrational combinations having excess and sub sufficient quantity of vitamin and minerals than recommended by Nutrional Guidelines of India. This observational study and analysis was done between April and September 16. Data was collected from current index of medical specialities and drug India. Rationality assessment was done using National list approved drug combinations by Central drug standard control organization (CDSCO) and essentially was cross checked using World Health Organization essential drug list 2015.Adequacy was analysed using Dietary reference intake (DRI). In our analysis, we have found 1184 irrational nutraceutical preparations available in India market. Out of 461(38.9%) are based on fat soluble vitamins, 190(16.4%) based on B-complex vitamins based and 5339(45.1%) related to essential minerals. Among 461 fat soluble vitamins, 104 contain excessive level, 334 contain sub standard levels. Similarly 128 and 62 Vitamin B-Complex based preparations are having excess and less quantity than recommended levels respectively and almost all the mineral combinations except four are not prepared following guidelines. None of these combinations were included in National essential list of medicines. Multivitamins are generally considered safe; these are irrationally prescribed and taken as self medication by public. Many of the ill effects are often unnoticed and under reported. Government of India should regulate the manufacture and sale these nutraceuticals to promote rational use of drugs and to promote well being and safety of Indian population which is primary objective of ‘Health for all’.
Maharashtra Udayagiri College, India
Time : 14:25-14:45
Karande B D is working as an Assistant Professor in Mathematics at Maharashtra Udaygiri College, Udgir Dist. Latur (INDIA). He presented his research papers in country like Thailand and Norway. Till today he has published 29 research papers in national and international Journals. he has also presented many research papers in national and international conferences. He is Life Member of Indian Science Congress, Indian Mathematical Society and Marathwada Mathematical Society.
Most of the natural and physical phenomena in the universe are not straight forward, since there is nonlinear nature of phenomenon in the area of sciences that are not continuous and involve jumps or discontinuity, such as effect of discontinuity or jumps of environment and heat stress on the agricultural food production, body growth, milk production, semen production, female reproduction of animals and also plant characters. Again, almost all such natural and physical phenomena involve the decay or growth, that is, the change in the state with respect to the time period. Increase in temperature and carbon dioxide can increase some crop yields in some places. The effects of climate change also need to be considered along with other evolving factors that effects agricultural food production. Agriculture is an important sector of the Indian economy. Agriculture and fisheries are highly dependent on the climate. Therefore, some of these types of problems may be formulated as nonlinear differential and integral equations involving discontinuous terms. Nonlinear differential and integral equations of arbitrary order play an important role in branch of nonlinear analysis and their applications in biological sciences. In this paper, we prove the existence solution or such type of nonlinear differential and integral equations and its applications. From the present investigation, it is concluded that the fixed point method which is powerful tool for existence solution of such type nonlinear differential and integral equations in Banach Spaces.
Teagasc Food Research Centre, Ireland
Title: Ultrasound assisted extraction of bioactive enriched fractions from button mushroom stalks waste
Time : 14:45-15:15
Bibha Kumari holds a degree in Food Processing and Technology from Central Food Technological Research Institute (CFTRI), Mysore, India. She has done her BSc in Agricultural Sciences. She is currently pursuing her PhD in Agriculture and Food Science from University College Dublin (UCD), Ireland. She is involved in a project titled, “The effect of novel processing on the levels of phytochemicals in brewer’s spent grains, mushroom stalks and potato peels”. Her research focuses on application of novel extraction technologies like ultrasonication and pulse electric field in bioactive extraction from plant sources. She has research interests in the area of Food Biotechnology.
Edible mushrooms possess interesting functional components like homo- and hetero- β-glucans [â(1→3), â(1→4) and â(1→6) glycosidic linkages], chitins, ergosterols, bioactive polysaccharides and peptides imparting health beneficial properties to mushrooms. Some of the proven biological activities are antioxidant, antimicrobial, immunomodulatory activities and cholesterol lowering activity by 3-hydroxy-3-methyl-glutaryl CoA reductase (HMGCR) inhibition. Application of novel extraction technologies like high power ultrasound offers clean, green, faster and efficient extraction alternatives with enhanced and good quality extracts. Ultrasound assisted extraction (UAE) was applied to recover bioactive enriched fractions from industrial white button mushroom (Agaricus bisporus) stalk waste using environmentally friendly and GRAS solvents i.e., water and water/ethanol combinations. The UAE treatment was carried out by placing 50 g of chopped fresh mushroom stalks and 75 ml of 80% ethanol using ultrasonic-water-bath (USB) of two frequencies (25 KHz and 35 KHz) for various treatment times (10, 20 and 30 min) at room temperature followed by 3 hours of agitation at 25ºC at 50 rpm in incubator shaker. The collected supernatant was filtered and characterized for its total proteins and phenolic content (TPC). USB with 25 KHz frequency with 30 min pre-treatment time had the highest TPC (15.65 mg GAE/g dry extract) with highest protein content of 1.65%. The freeze dried mushroom stalk powder was characterized for its compositional parameters (dry weight basis) showing 19.12% total protein, 7.21% total fat, 31.2% total dietary fiber, 7.9% chitin (as glucosamine equivalent) and 1.02% â-glucan content. Effect of ultrasonication on the recovery of crude polysaccharides and protein content with amino acid profile in different fractions will be presented.
Title: Halal industrialization into food production-global perspective, scientific gaps and proposed solutions
Time : 15:15-15:35
Raja N A Khan has his expertise in research, writing, planning and policy analysis regarding food and animal sciences. He is the author of two books and two research publications and producer of dozens of farmer extension TV program. As PhD student in Animal Sciences, he started research work on current emerging segregation in food industry, halal food. His research based on critical thinking and market trend analysis highlighted the gap areas in to halal food production, the key gap is none sufficient research and development approach.
Halal food is associated with billions of people with expected market worth 2.5 trillion USD at current and 4 as future estimation. It’s logical to predict that any problem with halal food can give birth to serious food-crises with huge losses to economics, market, food and energies along demand of huge shift of funds and food provision camps to cope it and cover up the need of food of halal user. If a food set up have some benefits then the same set up must have some drawbacks as well, especially when it’s not under scientific lines. Wise approach is to see the drawbacks, gaps and their expected solution as crises management relief. These gaps start from its definitions, conceptual level, production/farming, processing, certifications, marketing, consumer level, till sustainability. There is no or ineffective presence of any scientifically proven literature of halal standardization of products, halal food science, halal health, halal farming SOPs and halal R&D. Use of word “halal” as prefix with every industry and product other than food product from land to water without proper workout is not a wise approach as well. The absence of halal food grading system and halal standardization is the big hurdle in establishment of halal branding sustainably. A baseline scientific reference based on structural, function and nutritional profiles in determination of difference between Halal and Non-Halal are a vital and unavoidable question of future and market competition. The role of halal sharia expertise need to be well defined, standardize and lemmatized if halal food industry need to get develop on scientific lines as an open food of choice. Some research base definitions against following terminologies can be a good start toward development of actual halal science: halal sensory and chemical features, halal standardization, halal characterization, one unanimous halal definition and procedure, halal structural profile, halal nutritional profile, halal farming, halal scientific research & development. The outcome conclusions will serve as base line pilot study for many other studies.
Federal University of Agriculture, Nigeria
Title: The effect Of drying methods and varieties on carotenoid retention and functional properties of trifoliate yam (Dioscorea dumentorum) flour
Time : 15:35-15:55
Adegunwa M O is working as a Lecturer at Federal University of Agriculture, Abeokuta, Nigeria. She has extended her valuable service for many years and has been a recipient of many award and grants. Her international experience includes various programs, contributions and participation in different countries for diverse fields of study. Her research interests reflect in her wide range of publications in various national and international journals.
The effect of drying methods and varieties on the carotenoid retention, anti-nutritional factors and functional properties of trifoliate yam (Dioscorea dumetorum) flour were investigated. Flour was produced from yellow and white varieties of trifoliate yam using sun drying, solar drying, oven drying (40 oC), cabinet drying (40 oC). The pH, bulk density, dispersibility, water absorption index, oil absorption capacity, emulsion capacity ranged from 5.77 to 6.65; 0.66 to 0.76 g/ ml; 16.67 to 50.33 %, 135.47 to 189.87 %; 118.33 to 136.67 %, and 43.00 to 50.67 %, respectively. The effect of variety and drying method on the functional properties were significantly different (p < 0.05) except water binding capacity and foaming capacity. There were also significant differences in the effect of drying method and variety on the particle size distribution (p < 0.05) on the flours. The anti-nutritional factors showed that, different drying method reduced anti-nutrient compared to the raw tuber with values ranging from 5.83 to 13.15 % for alkaloid and 0.01 to 0.38 % for tannin. There were significant difference on the drying method and variety. The carotenoid content and retention was significantly affected by the drying method and variety with values ranging from 0.94 to 33.48 μg /g and 0.05 % to 0.34 %, respectively. Cabinet dried flour samples retained the highest amount of carotenoids while sun dried flours recorded the highest losses. The study showed that variety and drying had significant effect on the carotenoid retention, functional properties and anti-nutritional factors.
Sanjeevanee College Chapoli, India
Time : 16:20-16:40
Gaikwad S M did his PhD in Dairy Science. His expertise is in standardization of various traditional milk products. He is engaged in teaching, research and extension activities and has 13 years of teaching experience of graduate college. He has published 35 research papers in national and international journals and presented many research papers in national and international conferences. In 2015, he was felicitated with Young Scientist Award by SAADC, Pattaya, Thailand. He also won BOBLME travel award to participate in international conference and workshop held in Sri Lanka in the year 2014.
Lauz is a traditional dairy product specially prepared by Muslim community on the occasion of Eid. Very rarely it is prepared in some part of India, therefore very few people know about this product. It is prepared either by oven or deep frying in oil. The product is prepared by using suji (semolina), khoa, sugar, etc. As it is known to very few people nobody knows the correct method of its preparation. Therefore present investigation was carried out to standardize this concentrated deep fried or oven dried dairy product. Considering the initial investigation, the effect of three different levels of sugar, suji and khoa were studied on the sensory quality of this product. Sensory evaluation of fresh samples was done on a 9-point hedonic scale. It is observed that the control sample T1 got highest score (8.88) for the flavor as compared to the rest combinations. After this the sample T3 got highest score (8.66). For the body and texture the sample T1got highest score than the rest of samples. The body and texture of (T1) this sample also was appreciated by all the judges and might be due to this reason this sample awarded with highest score. As the suji quantity increased the body and texture score decreased. As the suji content increased the product got brownish color, therefore the panelists gave more to product. Therefore as the suji content increased the score also increased. From the present investigation it is concluded that the best quality of indigenous milk product lauz can be prepared by using 300 gm of khoa, 350 gm of suji, 300 gm of sugar and 50 gm of ghee for the combination of 1000 gm of product.
University of Malaya, Malaysia
Title: Amino acid biosensor based on L-amino acid oxidase immobilized onto Ag2O/CNT/ND/ Sago in Parkia speciosa juice
Time : 16:40-17:00
Natural food-derived peptides have attracted a great deal of interest among researchers due to the importance of a healthy diet. Parkia speciosa (stink bean), a Southeast Asian legume, is composed of medicinal chemicals which exhibit biological activities. Parkia speciosa has been reported to be anticancer, antibacterial, antioxidant, antiangiogenic and demonstrates hem-agglutinating activity. The compositional analysis of amino acids in Parkia speciosa seeds have been reported through hydrolysis using alcalase enzyme. Cyclic voltammetry (CV) using biosensor is a well-established technique with broad applications in nutrition analyses. A novel electrochemical Ag2O/CNT/ND biosensor, comprising silver-oxide, nano-diamond (ND) and carbon nanotube (CNT), has been fabricated on a copper sheet and used as the working electrode. In order to increase the stability of the biosensor, Sago, a natural biopolymer, was added to the composite. The Ag2O/CNT/ND and Ag2O/CNT/ND/Sago biosensors exhibited irreversible reaction free oxidation with reduction peaks at -1.25 and -1.16 V in 10 mM buffer phosphate solution/Parkia speciosa (pH 6.8), respectively. Amino acid biosensor was fabricated after the immobilization of L-amino acid oxidase on the Ag2O/CNT/ND/Sago electrode to estimate the level of amino acids in Parkia speciosa juice. The analysis of results suggested that the irreversible electro-chemical process was simultaneously adsorption and diffusion-controlled. The developed biosensor displayed a very good electro-catalytic activity toward the oxidation of amino acid to release H2O2 and NH3 as a result of the reaction between the active sites and the Parkia speciosa ingredient. This was also confirmed by a drop in the pH value from 6.8 to 6.55 and a change in the color of the solution from green to yellow. An increase in the charge transfer resistance at potentials higher than -1 V could be also explained by the co-formation of hydrogen peroxide (H2O2) and water on the electrode surface.
Soraya Hosseini has done her PhD in Chemical Engineering from University Kembangan Malaysia in 2010. It was then followed by a series of Post-doctoral positions at University Putra Malaysia from 2010 to 2016. Her PhD and the subsequent Post-doctoral research have led to about 45 research papers published in high-profile scientific journals in the field. She has been actively involved in environmental research and catalyst fabrication; however, her main research interest falls in the area of the fabrication of anhydrous membranes in fuel cell application. She has also been developing a growing interest in the area of advanced materials and electrochemical reactions. She has also conducted in-depth research on the fabrication of biosensors, employed in a range of food and energy. Her current research is concentrated around the fabrication of biosensors and probing into their performance by means of electrochemical reaction and impedance spectroscopy.